Secretory and glandular trichomes (SGTs) are found on the surfaces of above ground plant tissues of approximately one third of all vascular plant species. Humans are familiar with these structures because they contain the essential oils that give cooking herbs their distinctive smell and taste. In addition to making foods taste better, these uni- and multi-cellular appendages are proposed to play a critical role in plant protection against various environmental stresses including herbivore attack, pathogen infection, extreme temperature, and excessive light.
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Summer Internships 2015:
The tenth annual Plant Genomics @ Michigan State University Summer Undergraduate Research Program runs summer 2015. For information click here.
Recent publications from the project:
- NEWEST: Brasher MI , Surmacz L , Leong B , Pitcher J, Swiezewskab E, Pichersky E, Akhtar, TA. A two-component enzyme complex is required for dolichol biosynthesis in tomato. (2015) Plant J. Accepted Article, DOI: 10.1111/tpj.12859
- Schilmiller, A.L., G.D. Moghe, P. Fan, B. Ghosh, J. Ning, A.D. Jones, R.L. Last. 2015. Functionally divergent alleles and duplicated loci encoding an acyltransferase contribute to acylsugar metabolite diversity in Solanum Trichomes. The Plant Cell. http://dx.doi.org/10.1105/tpc.15.00087
- Ekanayaka, EAP, Celiz, MD and Jones, (2015). Relative mass defect filering of mass spectra: a path to discovery of plant specialized metabolites. Plant Physiol. 167: 1221-1232. doi: http://dx.doi.org/10.1104/pp.114
- Zi, J. Y. Matsuba, Y.J. Hong, A.J. Jackson, D.J. Tantillo, E. Pichersky and R.J. Peters 2014. Biosynthesis of lycosantalonol, a cis-prenyl derivedditerpenoid. J. Am. Chem Soc. DOI 10.1021/ja508477e
- Bedewitz, M.A., Gongora-Castillo, E., Uebler, J.B. Gonzales-Vigil, E., Wiegert-Rininger, K.E. et al. 2014. A root-expressed L-phenylalanine:4-hydroxyphenylpyruvate aminotransferase is required for tropane alkaloid biosynthesis in Atropa belladonna. Plant Cell 26:3745-3762. DOI 10.1105/tpc.114.130534
- Kim, J., Y. Matsuba, J. Ning, A.L. Schilmiller, D. Hammar, A.D. Jones, E. Pichersky and R.L. Last. 2014. Analysis of natural and induced variation in tomato glandular trichome flavonoids identifies a gene not present in the reference genome. Plant Cell. 26:3272-3285. doi:10.1105/tpc.114.129460.
- Wang, Z. and Jones, A.D. 2014. Profiling of stable isotope enrichment in specialized metabolites using liquid chromatography and multiplexed nonselective collision-induced dissociation. Anal. Chem. 86:10600-10607. doi: 10.1021/ac502205y
- Falara, V., Alba, J.M., Kant, M.R., Schuurink, R.C. and Pichersky, E. 2014. Geranyllinalool synthases in solanaceae and other angiosperms constitute an ancient branch of diterpene synthases involved in the synthesis of defensive compounds.Plant Physiol. 166:428-441. doi: 10.1104/pp.114.243246
- Kang, J.-H., J. McRoberts, F. Shi, J. E. Moreno, A. D. Jones, and G. A. Howe. 2014. The Flavonoid Biosynthetic Enzyme Chalcone Isomerase Modulates Terpenoid Production in Glandular Trichomes of Tomato.” PLANT PHYSIOLOGY 164: 1161-74.doi:10.1104/pp.113.233395.
For the full list of publications from this project click here.
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A compilation of NMR-resolved metabolite structures obtained by the project is available and will be updated as more confirmed structures are obtained. For access to this information click here.
Illumina sequencing reads for S. lycopersicum stem trichomes and shaved stems (depleted of trichomes) were deposited in the NCBI Sequence Read Archive under Bioproject PRJNA271503.
The Illumina sequencing reads for Atropa belladonna are available in the NCBI Sequence Read Archive under accession numbers SRX046282 to SRX046294, SRX060267, and SRX060269. Transcriptome assemblies and quantitative analysis of transcript abundance for the A. belladonnatranscriptome are available at http://medicinalplantgenomics.msu.edu/
LiveScience.Com piece on the AT2 gene. An article on the project can be found here.
RNA-seq data from trichome RNA isolated from seven S. habrochaites accessions is available in Genbank (SRX159744, SRX159100, SRX159765, SRX159766, SRX159767, SRX159768, and SRX159769). See Kim et al. (2012) for details.
S. lycopersicum trichome proteomics data now in GenBank.
2.3 Million project EST sequences available in GenBank.
ESTs of total trichomes of S. lycopersicum LA3475 (M82 cultivated tomato) are available as experiment SRX004021.
ESTs of type VI glands of S. lycopersicum LA3475 (M82 cultivated tomato) are available as experiment SRX004022.
ESTs of total trichomes of S. pennellii LA0716 are available as experiment SRX011590.
ESTs of total trichomes of S. habrochaites LA1777 are available as experiment SRX011591.
ESTs of type VI glands of S. habrochaites LA1777 are available as experiment SRX011592.
ESTs of type VI glands of S. pimpinellifolium LA1589 are available as experiment SRX011593.
ESTs of type VI glands of S. arcanum LA1708 are available as experiment SRX011594.
A total of 26,000 Sanger EST sequences from the project are available from GenBank. These are from S. habrochaites PI126449 type VI glands, S. habrochaites LA1777 types I and IV glands, S. lycopersicum LA3475 types I and VII and S. pennellii LA0716 types IV and VI glands.
Computer assembled consensus assemblies for these trichome ESTs are available for search here. Note that these contigs have not been assessed by human annotation or experimentally validated.